Biogenic Synthesis and Characterization of AgNPs Using CEPS: Cytotoxicity and Antibacterial Activites

This study was amid to biogenic synthesis of Silver Nanoparticles (AgNPs) and evaluated its chemical-physical biomedical properties. The currently used reducing agent is Polyalthia sclerophylla (CEPS). As a reducer, crude extract leaves (CEPS) used, while silver nitrate (AgNO3) as an initiator. Two samples were prepared named AgNPs-a AgNPs-b, respectively. carried out characterize their biological, physical, chemical Energy Dispersive X-Ray Analysis (EDX) ultraviolet-visible spectroscopy the first techniques utilised emphasise formulation AgNPs (Uv-vis). morphology size particles are determined using scanning transmission electron microscopy (STEM) (SEM). tested for cytotoxicity against Mg-63 human cells (a type osteosarcoma cell osteoblast-like cells) Alamar blue assay, antibacterial properties investigated Gram-positive Staphylococcus aureus (S. aureus) Gram-negative Escherichia coli (E. coli). wavelength 436 nm, AgNPs-b 441 according data. According SEM STEM images, shapes spherical. particle sizes not same, with having diameter range 48nm 68nm 59nm 77nm. availability in greater than 89% all concentrations. inhibited bacteria growth more effectively both bacteria, results S. Aureus E. at 80M/ml 24mm 22mm, respectively, compared same concentration 20mm 18mm. our obtained from techniques, similar, different. effect affects difference smaller inhibition bacterial growth. current has shown that non-toxic produced can be agent, nano-sizes employed safely medical biological areas.